Pre-Meeting Workshop

Pre-Advances Against Aspergillosis Workshop:

Aspergillus Speciation in the 21st Century -
Implications for Laboratory and Clinical Practice

Meliá Castilla Hotel and Conference Centre, Madrid, Spain

Tuesday 25 February 2014 : from 15.00 – 18.00
Wednesday 26 February 2014 : from 09.00 – 18.00

Supported by Gilead Sciences

Places are limited so early applications are recommenced to avoid disappointment.

Applicants should be medical doctors, scientists or clinical microbiologists or pharmacists, involved in diagnosis, prophylaxis and treatment of invasive fungal infections. Junior staff members are strongly encouraged to apply.


Please Note: Applications are no longer being accepted


Scientific Committee of Advances Against Aspergillosis
Instituto de Salud Carlos III (Centro Nacional de Microbiología)


Course Objectives

The genus Aspergillus contains approximately 250 species, divided into eight subgenera which are then subdivided into several sections or species complexes. With the use of molecular tools and taxonomic studies, it is now clear that some species are actually a complex of various species that are morphologically similar and have been misidentified for years.

Recent studies have shown that sibling/cryptic Aspergillus species seem to be underestimated, since they can cause approximately 10% of the cases of invasive aspergillosis and be involved in many mixed infections. In addition, correct classification is clinically relevant as 40% of isolates belonging to those cryptic species exhibit in vitro resistance to one or more antifungal compounds.

During this course, we will review the current status of several diagnostic and clinical aspects of infections by these important Aspergillus species.

The specific objectives of the workshop are:

  • Review the present situation of the epidemiology of sibling/cryptic species of Aspergillus
  • Evaluate conventional methods and new techniques for identification of these species
  • Learn appropriate methods for in vitro antifungal susceptibility testing, including commercial techniques
  • Learn criteria for prophylaxis and treatment of invasive fungal infections caused by these cryptic/sibling species
  • Learn about recommendations for routine daily laboratory and clinical practice
  • Define priorities and exchange experiences on how to coordinate microbiological data and clinical practice

Course Outcomes

At the end of this course, you will be able to:

1.   Be conversant with Aspergillus taxonomy and the importance sibling/cryptic species of Aspergillus
2.   Know the common antifungal resistant patterns for important and emerging cryptic species
3.   Be comfortable about how to identify to genus (section), complex or species level, and how the clinical laboratory should be reporting Aspergilli.
4.   Be aware of the clinical complexities associated with infection with unusual species such as Aspergillus calidoustus, A. lentulus, and N. pseudofisheri.

Workshop Coordinator

Manuel Cuenca-Estrella, MD PhD
Mycology Department of Spanish National Center for Microbiology
Instituto de Salud Carlos III, Majadahonda, Spain


José Maria Aguado, MD PhD
Hospital Universitario 12 de Octubre, Spain

Ana Alastruey-Izquierdo, PhD
Instituto de Salud Carlos III, Spain

Laura Alcazar-Fuoli, PhD
Instituto de Salud Carlos III, Spain

Manuel Cuenca-Estrella, MD PhD
Instituto de Salud Carlos III, Spain

David W. Denning, FMedSci
University of Manchester, UK

Jesús V. Guinea, PhD
Hospital General Universitario Gregorio Marañón, Spain

Mohammad T. Hedayati, PhD
Mazandaran University of Medical Sciences, Iran

Susan J. Howard, PhD
University of Liverpool, UK

Cornelia Lass-Flörl, MD PhD
Innsbruck Medical University, Austria

Emilia Mellado, MD PhD
Instituto de Salud Carlos III, Spain

Patricia Muñoz, MD PhD
Hospital General Universitario Gregorio Marañón, Spain

Alessandro Pasqualotto, MD PhD
Universidade Federal de Ciências de Saúde de Porto Alegre (UFCSPA), Brazil

Jan W.M. van der Linden, MD
Radboud University Nijmegen Medical Center, The Netherlands

Janos Varga, PhD
University of Szeged, Hungary



Tuesday 25 February


Manuel Cuenca-Estrella


Current classification of the clinically relevant Aspergillus species complexes
Cornelia Lass-Flörl


Methods for identification: conventional, DNA sequencing, MALDI-TOF
Mohammad Hedayati


Diagnostic methods to detect Aspergillus species in clinical samples using conventional and non-culture techniques
Jesus Guinea




Workshop social evening and dinner


Wednesday 26 February


Epidemiology of infections by cryptic species of Aspergillus: TRANSNET, SCARE Multicenter Study, FILPOP survey
Jan W.M. van der Linden


In vitro antifungal susceptibility of cryptic Aspergillus species
Ana Alastruey-Izquierdo


Clinical profile of cryptic Aspergillus species
David W. Denning




Treatment of infections by cryptic Aspergillus species
Alessandro Pasqualotto


Visual session of unusual Aspergillus species: images and plates
Janos Varga




Case studies (clinical and microbiological) 45 minutes each
Chairman: Jose M. Aguado

  • Diagnosis and treatment of aspergillosis (mixed infection with cryptic species)
    Patricia Muñoz
  • Multi-resistant aspergillosis due to cryptic species
    Susan Howard
  • Study of antifungal resistance mechanisms in cryptic species
    Emilia Mellado
  • In vivo response in a mixed infection model of azole susceptible and resistant Aspergillus spp. strains
    Laura Alcazar-Fuoli
  • Recommendations for a routine daily practice
    Manuel Cuenca-Estrella


Closing remarks
David W. Denning

Tuition Fee

The tuition fee will be £100. This will include:

  • Attendance at the workshop
  • Tea/coffee throughout the event
  • Dinner on Tuesday 25 February (following the end of the first session)
  • Lunch on Wednesday 26 February

Accommodation and travel are not included.


Accommodation at the Meliá Castilla Hotel can be booked on the Registration page (link to Registration page please) of the conference website.
If you require any further information about this, please contact


Please click here to submit your application for a place on the workshop.

Notification of the outcome of applications will be sent by email within 2 weeks.

To confirm their place, successful applicants will then be required to pay the registration fee via the conference website. Detailed information about this process will be given in the confirmation email.

Workshop Language

The official language of the workshop will be English with no simultaneous translation.

Course Secretariat

Derry Brothers
Hartley Taylor Medical Communications Ltd
Caledonian House, Tatton Street, Knutsford, Cheshire, WA16 6AG, UK
Email: or telephone: +44 (0)1565 621697

Spanish Secretariat Coordinators

Dr Laura Alcazar-Fuoli (
Dr Ana Alastruey-Izquierdo (
Tel: +34 91 8223661